Figure 1
From: Microtubules Modulate F-actin Dynamics during Neuronal Polarization
Drebrin promotes microtubule entry into growth cones. (a–c) Drebrin-YFP expression promotes entry of EB3 comets to the peripheral domain of growth cones compared to growth cones expressing Lifeact-GFP (kymographs from white line). (d) Quantification of growth cone area occupied by EB3 comets. In percentage (%), EB3-mCherry/Lifeact-GFP cells = 52.12 ± 2.182; n = 10 cells from at least three different cultures; EB3-mCherry/Drebrin-YFP cells = 76.51 ± 2.019; n = 10 cells from at least three different cultures; ****p < 0.0001 by t test; Mean ± SEM (e) Representative stage 2 cell transfected with Drebrin-YFP and EB3-mCherry showing the neurite with higher Drebrin intensity receives more EB3 comets. The neurite (n1) that is enriched with overexpressed Drebrin (white arrow) received higher number of EB3 comets (see the kymograph from white lines, n1–n5) (f) Pearson correlation analysis of Drebrin-YFP intensity and EB3 comets number entering into the neurites (per 5 min) from stage 2 cells. n = 8 cells from at least three different cultures; Values were normalized according to standard score and axes are represented in units of standard deviation [σ]. Line equation: Y = 0.6698*X + 5.742e-008; Pearson r = 0.6698, p < 0.0001. Dashed lines represent 95% confidence intervals. Scale bar: 10 μm (e).
