Figure 3
From: Microtubules Modulate F-actin Dynamics during Neuronal Polarization
Drebrin localization predicts the position of axon outgrowth. (a) Stage 2 and stage 3 neurons transfected with Drebrin-YFP and Lifeact-RFP showing polarized Drebrin signal at one neurite tip. (b) Quantifications showing percentage of stage 2 and stage 3 cells with polarized Drebrin signal. In percentage %, stage 2 = 49.21 ± 6.349, stage 3 = 69.57 ± 6.859, stage 2 cells n = 63, stage 3 cells n = 46, p = 0.0337, Student’s t test, *p < 0.05; Mean ± SEM. (c) Neuron expressing Drebrin-YFP and Lifeact-RFP forms an axon from the dynamic neurite, which shows more Drebrin-YFP (d) Tau-1 staining confirms the axonal identity of the cell. Scale bar: 10 μm (a and c).
