Figure 8
From: Microtubules Modulate F-actin Dynamics during Neuronal Polarization
Microtubule/Drebrin interaction affects F-actin polymerization in vitro. (a) Kinetics of in vitro reconstitution assay of actin alone and in the presence of, actin with Drebrin or DrebrinS142D and actin, microtubules, EB3, Drebrin or Drebrin S142D. Curves represent mean values obtained from 2–5 independent experiments. (b) Ymax values are calculated from 2–5 independent experiments. Y max values for Actin alone = 1.035 ± 0.04785 A.U.; Actin + Dbn = 0.7504 ± 0.03016; Actin + DbnS142D = 0.8003 ± 0.0116 A.U; Actin + MTs + EB3 + Dbn = 0.6915 ± 0.06875 A.U. Actin + MTs + EB3 + DbnS142D = 0.5267 ± 0.06545 A.U.; *p < 0.05, **p < 0.01, ***p < 0.001 by one-way ANOVA, post hoc Dunnett’s test; Mean ± SEM. (c) Representative TIRF images of in vitro actin polymerization assay under specified conditions. F-actin (labeled by Alexa-Fluor 488 phalloidin) is shown in green and microtubules (MT, labeled by HyLight647) are shown in red. Scale bar: 10 µm.
