Figure 6 | Scientific Reports

Figure 6

From: Phospholipase D2 loss results in increased blood pressure via inhibition of the endothelial nitric oxide synthase pathway

Figure 6

Recovery of eNOS levels in shPLD2 cells following exogenous cholesterol supplementation. (A) Free cholesterol levels monitored by cholesterol oxidase assay after culture of cells with 25 μM exogenous cholesterol (in media) for 24 hours (n = 4). (B) Representative western blot of eNOS protein in shPLD2 cells with and without cholesterol supplementation. The regions of the Western blots (Suppl. Figure 3) containing the eNOS and actin immunoreactive bands were scanned and the relative abundance of the individual samples quantified using an Odyssey CLx imaging system. (C) Quantification of western blot with eNOS levels normalized to actin (n = 5). (D) Schematic summary of model. Dotted line indicates that the mechanism involved is unknown at this time. Mean ± SEM; *p < 0.05; **p ≤ 0.001; C, Student’s t-test; A, one-way ANOVA with Bonferroni’s Multiple Comparison Test.

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