Table 1 Orders of nonlinearity for STED-light-induced fluorescence, photobleaching and photobleaching by STED light on its own, as measured by varying the STED pulse duration over the range 0.1–3 ps.

From: Photobleaching in STED nanoscopy and its dependence on the photon flux applied for reversible silencing of the fluorophore

 

STED-light-induced fluorescence (sf)

Bleaching (b)

Bleaching by STED only

ATTO647N

1.52 ± 0.02

1.39 ± 0.02

1.86 ± 0.05

ATTO590

2.15 ± 0.07

1.89 ± 0.06

3.02 ± 0.08

STAR635P

1.73 ± 0.02

1.35 ± 0.02

2.03 ± 0.04

STAR580

1.92 ± 0.05

1.66 ± 0.04

2.40 ± 0.07

  1. Tabulated coefficients are retrieved from line slopes presented in Fig. 2b–d, also shown in Fig. 3b,c. Time-averaged STED power: 10 mW (Ep = 0.125 nJ).
Back to article page