Figure 5 | Scientific Reports

Figure 5

From: Exosomes from high glucose-treated glomerular endothelial cells trigger the epithelial-mesenchymal transition and dysfunction of podocytes

Figure 5

Exosomes from cells undergoing the EndoMT increase TGF-β1 expression and activate Wnt/β-catenin signaling in podocytes. (A) RT-PCR and Western blot analysis indicated that exosomes from HG-treated GECs increased TGF-β1 expression in cultured podocytes. The results are presented as the mean ± SD. *p < 0.05 (ANOVA; n = 3; significantly different from the Untreated group and NG-GEC-Exo group). (B) RT-PCR demonstrated an increased expression of Wnt1 mRNA in cultured podocytes after treatment with exosomes from HG-treated GECs. *p < 0.05 (ANOVA; n = 4; significantly different from the Untreated group and NG-GEC-Exo group). (C, D) Western blot analysis and immunofluorescence staining showed that exosomes from HG-treated GECs increased total β-catenin, active β-catenin and Snail protein expression and promoted β-catenin nuclear translocation in podocytes. The results are presented as the mean ± SD. *p < 0.05 (ANOVA; n = 3; significantly different from the Untreated group and NG-GEC-Exo group).

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