Figure 6 | Scientific Reports

Figure 6

From: Exosomes from high glucose-treated glomerular endothelial cells trigger the epithelial-mesenchymal transition and dysfunction of podocytes

Figure 6

Inhibition of exosomal TGF-β1 preserves podocyte phenotypes. (A) HG increased TGF-β1 mRNA expression in both GECs and related exosomes. The results are presented as the mean ± SD. *p < 0.05 (Student’s t-test; n = 3; significantly different from the NG group and NG-GEC-Exo group). (B) A remarkable decrease in TGF-β1 mRNA expression was observed in HG-treated GECs incubated with TGF-β1 siRNA and related exosomes. The results are expressed as the mean ± SD. *p < 0.05 (ANOVA; n = 3; significantly different from the NG group and NG-GEC-Exo group). **p < 0.05 (ANOVA; n = 3; significantly different from the HG group and HG-GEC-Exo group). (C) Compared to expression in the HG-GEC-Exo group, TGF-β1, β-catenin, active β-catenin, Snail, α-SMA, FSP-1, and desmin expression decreased and nephrin, ZO-1, and WT1 expression increased markedly in the podocytes incubated with exosomes silenced for TGF-β1. The results are presented as the mean ± SD. *p < 0.05 (ANOVA; n = 3; significantly different from the Untreated group). **p < 0.05 (ANOVA; n = 3; significantly different from the HG-GEC-Exo group).

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