Figure 2

Delphinidin inhibits T lymphocyte proliferation through SOCE-dependent pathway. (A) Histograms show the percentage of proliferation of cells exposed to either 10⁻² g/L of delphinidin (Del), 5 µg/mL PHA or both, in absence or in presence of 10 µM of SOCE inhibitor (SKF96365) for 48 h. (B) Representative traces (left) and histograms (right) showing the effect of 10⁻² g/L of delphinidin (Del) alone or after activation by 1 µM thapsigargin (TG) on [Ca²⁺]_i in Ca²⁺-containing PSS. (C) Same experiments in presence of 10 µM of SOCE inhibitor (SKF96365). (D) Representative traces (left) and histograms (right) showing the effect of 10⁻² g/L delphinidin on [Ca²⁺]_i increase induced by 1.25 mM of CaCl₂ after depletion of intracellular stores in Ca²⁺-free PSS by 1 µM TG. (E) Same experiments in presence of 10 µM of SOCE inhibitor, SKF96365. Data are the mean ± SEM (n = 7–11). *P < 0.05, **P < 0.01 and ***P < 0.001 versus control group. ^# P < 0.05 and ^## P < 0.01 versus TG group.