Figure 5 | Scientific Reports

Figure 5

From: Estrogen receptor α/HDAC/NFAT axis for delphinidin effects on proliferation and differentiation of T lymphocytes from patients with cardiovascular risks

Figure 5

Effect of delphinidin on the differentiation of T lymphocytes from healthy subjects. (A) Cells were stimulated for 24 h with 10−2 g/L of delphinidin (Del), 5 µg/mL of PHA or both and stained for T-bet, GATA3, RORγt and FOXP3 transcription factors. Histograms show the percentage of positive cells for T-bet, GATA3, RORγt and FOXP3 expressions. Data are the mean ± SEM (n = 7). (B) T cells were stimulated for 5 h with 10−2 g/L of delphinidin (Del), 50 ng/mL phorbol-12-myristate-13-acetate (PMA) plus 1 µg/mL ionomycin (Ion) or both, in the presence of 5 µg/mL brefeldin A (BFA) for the last 3 h of culture and stained for IL-2, IFNγ, IL-4, IL-17A and IL-10 cytokines. Histograms show the MFI of positive cells for IL-2, IFNγ, IL-4, IL-17A, and IL-10, respectively. Data are the mean ± SEM (n = 7). *P < 0.05, **P < 0.01 and ***P < 0.001.

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