Figure 2
From: Optogenetic regulation of insulin secretion in pancreatic β-cells
Insulin secretion by β-cells expressing bPAC. (A) MIN6 β-cells expressing bPAC displayed higher rates of insulin release (ng insulin/mg total protein/h) upon exposure to light compared to cells kept in dark and non-transduced cells or cells infected with AdGFP. MOI = 100; illumination for 30 min; *p < 0.05 vs. all other samples at 25 mM glucose; #p < 0.05 vs. all other samples at 2.5 mM glucose. (B) [cAMP]i and insulin secretion for MIN6 β-cells in the absence of glucose (MOI = 100); *p < 0.05 vs. all other samples. (C) Insulin secretion was determined in MIN6 β-cell monolayers (without bPAC expression) treated with various secretagogues at 2.5 or 25 mM glucose (see Materials and Methods). Results are shown as mean ± SD from at least three independent experiments.
