Figure 4
From: Optogenetic regulation of insulin secretion in pancreatic β-cells
Expression of bPAC by murine islets and MIN6 β-cell PIs. (A) [cAMP]i content and (B) insulin secretion rate of murine islets without transduction (control) or transduced with AdbPAC or AdGFP. All islets were incubated with the stated concentration of glucose while exposed to blue light. *p < 0.01 AdbPAC-transduced vs. control islets in corresponding conditions. **p < 0.01, AdbPAC-transduced islets at 20.2 mM vs. 2.8 mM glucose. (C) Micrographs of PIs of MIN6 β-cells transduced with AdbPAC. Brightfield and epifluorescence (mCherry) images are shown. Bar: 100 µm. (D) The rate of insulin release by PIs of MIN6 β-cells expressing bPAC was higher upon illumination (30 min) with blue light (bPAC + light; MOI = 100) compared to PIs of regular MIN6 cells (control). *p < 0.05. For forskolin treatment: **, ***p < 0.05 vs. control and bPAC + light at 2.5 mM or 25 mM glucose, respectively. Treatment with IBMX, tolbutamide or KIC resulted in significantly higher release rates than those of the control samples (control; p < 0.05) for the corresponding glucose concentration but similar to those of PIs expressing bPAC and exposed to light.
