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Figure 1

From: Synergistic impact of mutations in Hepatitis B Virus genome contribute to its occult phenotype in chronic Hepatitis C Virus carriers

Figure 1

Effects of substitutions in ORF-S on HBsAg antigenicity and its detection. (A) Antigenicity plot of “a” determinant (aa.124–147) of HBsAg showing decreased antigenicity due to substitutions T125M and P127T as obtained by Kolaskar and Tongaonkar Antigenicity Prediction method. The relative ratio of PreS2/S and PreS1 mRNA determined by quantitative real-time PCR (B), the expression of HBV envelope proteins by immunoblot assay with mouse monoclonal anti-HBs primary antibody [the cropped gels are shown here for clarity while the full-length blots are presented in Supplementary Figure S2 (A); α-Tubulin served as the loading control] (C) and HBsAg level [Signal(S)/Cutoff(CO)] in culture supernatant estimated by ELISA (D), following transfection of wt-HBV (HBV/D-wt) and mutant construct [HBV/D-mt(HBsAg)], having T125M and P127T substitutions in Huh7 cells. Paired t-test p values; ***p < 0.0005, ns; non-significant.

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