Figure 3 | Scientific Reports

Figure 3

From: Synergistic impact of mutations in Hepatitis B Virus genome contribute to its occult phenotype in chronic Hepatitis C Virus carriers

Figure 3

Effects of substitutions in Enh-II on enhancer activity, pgRNA expression and HBV-DNA level. (A) Relative luciferase activities of the constructs pGL3-P-wt-Enh-II and pGL3-P-mt-Enh-II carrying wild-type and mutant Enh-II elements respectively in pGL3-promoter vector. Relative expression of pgRNA (B), intracellular HBV-DNA level (C) and extracellular HBV-DNA level (D), as measured by quantitative real-time PCR, following transfection of the full-length, wild-type HBV/D (HBV/D-wt) and corresponding Enh-II-mutated HBV [HBV/D-mt(Enh-II)] in Huh7 cells. Paired t-test p values; *p < 0.05, **p < 0.005, ***p < 0.0005.

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