Figure 4 | Scientific Reports

Figure 4

From: Synergistic impact of mutations in Hepatitis B Virus genome contribute to its occult phenotype in chronic Hepatitis C Virus carriers

Figure 4

Effects of substitutions in Enh-II on the envelope protein expression. Relative ratio of PreS2 and PreS1-mRNA as determined by quantitative real-time PCR (A), expression of HBV envelope proteins by immunoblot assay with mouse monoclonal anti-HBs primary antibody [the cropped gels are shown here for clarity while the full-length blots are presented in Supplementary Figure S2 (B); α-Tubulin served as the loading control] (B) and HBsAg level [Signal(S)/Cutoff(CO)] in culture supernatant estimated by ELISA (C), following transfection of the full-length, wild-type HBV/D (HBV/D-wt) and corresponding Enh-II-mutated HBV [HBV/D-mt(Enh-II)] in Huh7 cells. Paired t-test p values; *p < 0.05.

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