Figure 5 | Scientific Reports

Figure 5

From: Synergistic impact of mutations in Hepatitis B Virus genome contribute to its occult phenotype in chronic Hepatitis C Virus carriers

Figure 5

Effects of substitutions in X-promoter on promoter activity, pgRNA expression and HBV-DNA level. (A) Relative luciferase activities of the constructs pGL3-B-HBx-promoter-wt and pGL3-B-HBx-promoter-mt carrying wild-type and mutated X-promoter respectively cloned in pGL3-Basic vector. Relative expression of pgRNA (B) and intracellular HBV-DNA level (C) as measured by quantitative real-time PCR, following transfection of the full-length, wild-type HBV/D (HBV/D-wt) and corresponding X-promoter-mutated HBV [HBV/D-mt(X-prom)] in Huh7 cells. Paired t-test p values; *p < 0.05, **p < 0.005, ***p < 0.0005.

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