Table 2 Frequencies of mutations across the complete genome of OHBV/D as compared to HBV/D from HBsAg-positive carriers.
Site in HBV Amino Acid/Nucleotide Substitution | OHBV/D sequences determined in this study (n = 17)(%) | HBV/D sequences from HBsAg-Positive carriers of Eastern India (EI) (n = 62)(%) | HBV/D sequences from HBsAg-Positive carriers of other geographical regions (WRLD) (n = 105)(%) | p2 (OHBV vs HBV/D_EI)a | p1 (OHBV vs HBV/D_WRLD)a |
|---|---|---|---|---|---|
PreS1/PreS2/S-ORF | |||||
PreS2-P36L | 11(64.9) | 10(16) | 1(0.9) | 1.97E-04 | 1.01E-10 |
PreS2-I42L | 14(82.6) | 16(25.6) | 27(25.6) | 3.59E-05 | 1.25E-05 |
P36L+I42L | 11(64.9) | 9(14.4) | 1(0.9) | 1.03E-04 | 1.01E-10 |
sT125M | 16(94.4) | 22(35.2) | 20(19) | 1.37E-05 | 2.48E-09 |
sP127T | 17(100) | 43(68.8) | 39(37) | 8.29E-03 | 3.82E-07 |
sT125M+sP127T | 16(94.4) | 21(33.6) | 20(19) | 1.02E-05 | 2.48E-09 |
X-ORF | |||||
R26C | 11(64.9) | 11(17.6) | 19(18) | 3.57E-04 | 1.70E-04 |
P33S | 11(64.9) | 19(30.4) | 34(32.3) | 2.19E-02 | 1.46E-02 |
R26C+P33S | 11(64.9) | 11(17.6) | 10(9.5) | 3.57E-04 | 1.84E-06 |
ORF-P | |||||
spE1D | 12(70.8) | 10(16.1) | 13(12.4) | 3.65E-05 | 1.38E-06 |
spI59F | 10(59) | 13(20.9) | 22(20.9) | 5.17E-03 | 2.23E-03 |
spL80F | 11(64.9) | 14(22.5) | 35(33.3) | 2.26E-03 | 1.68E-02 |
spL116F | 10(59) | 10(16.1) | 13(12.3) | 8.92E-04 | 7.26E-05 |
rtA21S | 11(64.9) | 15(24.2) | 26(24.7) | 3.01E-03 | 2.97E-03 |
rtF122L | 10(59) | 14(22.5) | 24(22.8) | 6.87E-03 | 6.33E-03 |
rtH124N | 13(76.7) | 9(14.5) | 5(4.7) | 2.58E-06 | 1.56E-10 |
rtQ130P | 10(59) | 17(27.4) | 22(20.9) | 2.19E-02 | 2.23E-03 |
rtN131D | 10(59) | 9(14.5) | 4(3.8) | 4.97E-04 | 1.11E-07 |
rtD263E | 14(82.6) | 28(45.1) | 40(38) | 1.20E-02 | 1.04E-03 |
rtV278I | 11(64.9) | 8(12.9) | 14(13.3) | 5.10E-05 | 4.57E-05 |
rhL30S | 11(64.9) | 7(11.3) | 10(9.5) | 2.35E-05 | 1.84E-06 |
rhL55I | 14(82.6) | 27(43.5) | 38(36.1) | 5.79E-03 | 4.53E-04 |
X-Promoter | |||||
T1050G | 12(70.6) | 12(19.4) | 12(11.4) | 1.29E-04 | 7.52E-07 |
A1053G | 11(64.7) | 11(17.7) | 10(9.5) | 3.57E-04 | 1.84E-06 |
C1059T | 11(64.7) | 13(21) | 14(13.3) | 1.89E-03 | 1.85E-05 |
C1350A | 11(64.7) | 8(12.9) | 2(1.9) | 5.10E-05 | 6.21E-10 |
Enh-II | |||||
C1637A | 11(64.7) | 9(14.5) | 9(8.6) | 1.03E-04 | 9.23E-07 |
T1676A | 12(70.6) | 13(21) | 7(6.7) | 2.28E-04 | 1.77E-08 |
