Figure 4 | Scientific Reports

Figure 4

From: Metabolomic Analysis Reveals Vitamin D-induced Decrease in Polyol Pathway and Subtle Modulation of Glycolysis in HEK293T Cells

Figure 4

Vitamin D treatment induces a decrease in enolase and an increase in PGM activities. To perform enzymatic assays, HEK293T cells were treated for 24 hours with 100 nM of vitamin D or vehicle before cell lysis. The activity of glycolytic enzymes was assayed as described in methods. The graph indicates the fold change on the activity of each enzyme after vitamin D-treatment in relation to vehicle treatment (control). Values represent average ± SD; n = 6 for HK, n = 4 for PGM, n = 7 for Eno and n = 3 for the other enzymes. HK = hexokinase; HPI = hexose phosphate isomerase; PFK = phosphofructokinase; ALDO = aldolase; TPI = triose phosphate isomerase; GAPDH = glyceraldehyde 3-phosphate dehydrogenase; PGK = phosphoglycerate kinase; PGM = phosphoglycerate mutase; Eno = enolase; PK = pyruvate kinase. For the analysis of the statistical significance with and without vitamin D we used unpaired t-test (**, ***p < 0.05).

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