Figure 6 | Scientific Reports

Figure 6

From: Metabolomic Analysis Reveals Vitamin D-induced Decrease in Polyol Pathway and Subtle Modulation of Glycolysis in HEK293T Cells

Figure 6

Vitamin D modulate expression of important genes. HEK293T cells were treated for 24 hours with 100 nM vitamin D, and total RNA were extracted followed by RT-qPCR. TXNIP and TRX, are linked to oxidative stress. ASK1 is linked to apoptose. MCT1/4 are linked to lactate exportation. GLUT1/3 are linked to glucose transport. HK1/2 and LDHa/b are linked to glycolysis. GLS1/2, GS and GDH are linked to glutamine metabolism. Each bar corresponds to the triplicate mean fold change relative to β-actin gene. Error bars correspond to SD of biological triplicate. Statistical significance was evaluated using unpaired t-test (*p < 0.05) according Material and Methods.

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