Figure 2
From: Holding of bovine blastocysts at suprazero temperatures using small molecules
The effects of small molecules on bovine embryo incubation at 20 °C for 72 h. (a) After incubation at 20 °C for 72 h, the morphology of the embryos incubated in three different small molecule groups was comparable to those incubated in BNC, appearing dark and shrunken. (b) After in vitro culture for 48 h (recovering period), expanded and hatched embryos were shown in Y−, C+Y− and C+T-BNC media with no morphological differences. However, there were no living embryos after incubation in BNC. Abbreviations are the same as in Table 2. Scale bar = 100 μm.
