Figure 2 | Scientific Reports

Figure 2

From: Activation-tagging in indica rice identifies a novel transcription factor subunit, NF-YC13 associated with salt tolerance

Figure 2

Physical mapping of insertion site of Ds-Enhancer (DS-En) elements and expression analysis of NF-YC13 in salt tolerant activation-tagged DS-16 line. (A) Ds element was inserted in the intergenic region on chromosome 1 and location of neighboring four putative genes (up to 20 kb region) near the insertion site. (B) Semi-quantitative RT-PCR analysis of activation-tagged DS-16 line. Two weeks old seedlings grown in Yoshida’s solution supplemented with 150 mM NaCl for 15 d and those without NaCl supplementation used as control. One of the genes, LOC_Os01g08790 shows expression during control and NaCl stress conditions while no expression in leaf and root of WT-BPT and LOC_Os01g08800 respectively. (C,D) Quantitative real time-PCR analysis of expression of NF-YC13 in the activation-tagged DS-16 line in leaf and root tissues. OsActin1 was used as a reference gene for quantitative RT-PCR. Means of three independent samples and standard errors are presented. ** and * indicates significant difference at P < 0.01 and P < 0.05.

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