Figure 4
From: Establishment and genomic characterization of the new chordoma cell line Chor-IN-1
NGS and aCGH copy number profiles on the different chordoma cell lines.Copy number profiles of Chor-IN-1 cell line vs. the other chordoma cell lines obtained by NGS and aCGH analysis: (a) NGS analysis: the different cell lines were profiled by NGS using Illumina TruSight One “clinical exome” panel (Illumina, San Diego, CA, USA). Log2 ratio of the coverage calculated in each interval vs. medium coverage is reported in the plot. (b) aCGH analysis: the different cell lines were profiled by aCGH. The Array Comparative Genomic Hybridization (aCGH) characterization was performed using the oligonucleotide-based microarray CytoChip Cancer 8 × 60 kv2.0 for Chor-IN-1, U-CH1 and U-CH2 samples and CytoChip ISCA 8 × 60 K v2.0 for MUG-Chor1 and JHC7 samples (BlueGenome Ltd, Cambridge, UK). Log2 ratio of cell line (ch1) vs. reference (ch2) value was calculated. Regions with chromosomal gain (log2 ratio > 0.2) are reported in green, while regions of chromosomal loss (log2 ratio < −0.2) are reported in red. Balanced genomic regions are reported in grey.
