Figure 1

Protoplast preparation and transformation for Nodulisporium sp. (No. 65-12-7-1). (a) The amount of protoplasts produced by treatment of the mycelia of Nodulisporium sp. (No. 65-12-7-1) with 3 different enzymes: Yatalase (10 mg/mL), Driselase (10 mg/mL) and Snailase (10 mg/mL). (b) Microscopic check of protoplasts released from the mycelium of Nodulisporium sp. (No. 65-12-7-1). (c) The amount of protoplasts produced by Yatalase treatment for different incubation time. (d) The regeneration frequency of protoplasts produced by Yatalase treatment for different incubation time. (e) The amount of protoplasts produced from the mycelia of different age. (f) The regeneration frequency of protoplast produced from the mycelia of different age. (g) The transformation efficiency under different concentrations of PEG. Student’s t-test was performed for comparing the transformation efficiency under different concentrations of PEG. (h) The transformation efficiency under different concentrations of Ca²⁺. All values are means ± standard error of the mean from three independent experiments.