Figure 2 | Scientific Reports

Figure 2

From: A novel automatic quantification method for high-content screening analysis of DNA double strand-break response

Figure 2

Automatic foci segmentation with fuzzy c-means clustering. (a) γH2AX foci image of a MCF-7 cell nucleus randomly chosen from the control group. (b) Gray level image of (a). (c) Gray level image of the first cluster after processing with fuzzy c-means clustering. (d) Gray level image of the second cluster after processing with fuzzy c-means clustering. (e) Gray level image of the third cluster after processing with fuzzy c-means clustering. (f) Three-dimensional display of (b). (g) Three-dimensional display of (c). (h) Three-dimensional display of (d). (i) Three-dimensional display of (e). (f*–i*) Two-dimensional pseudo-images of (f–i).

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