Figure 3
Results of automatic γH2AX foci quantification for images of MCF-7 and A549 cells from high-content screening. Cells incubated with DMSO and/or MS-275 (in DMSO) were exposed to 10-Gy radiation and then were cultured for another 1 hour before immunostained with antibodies against γH2AX and 53BP1. Quantification was performed using three different quantitative methods. (a) (a) Whole visual field of cells treated with DMSO (MCF-7). (b) Whole visual field of cells treated with MS-275 (in DMSO). (c) Single cell nucleus image from cells treated with DMSO. (d) Single cell nucleus image from cells treated with MS-275 (in DMSO). (b) (a) Quantification of γH2AX foci numbers. (b) Quantification of γH2AX foci areas. (c) Quantification of γH2AX foci intensity. Error bars represent standard deviation (SD) of three independent experiments, each based on more than 100 cells under each treatment condition. Significant differences were observed compared to DMSO-treated cells, and the p values of all quantitation methods were the same (p = 0.000). The data were derived from three independent experiments. **p < 0.01.
