Figure 5
Mid- and long-term effects of Rot, 2-DG, ATQ, PQ, compound 1o and ML304 on the redox ratio of P. falciparum NF54[roGFP2-Orp1]-attB and NF54[Mito-roGFP2-Orp1]-attB-transfected parasites. (a) 4 h incubation of NF54[roGFP2-Orp1]-attB transfectants with the compounds led to an increase of fluorescence ratio for both sensors as determined using CLSM. ML304 in particular significantly increased the 405/488 nm ratio of roGFP2-Orp1. (b) 24 h incubations with the drugs seemed to have a higher influence on the fluorescence ratio for NF54[Mito-roGFP2-Orp1]-attB transfectants, in which compound 1o significantly increased the mitochondrial probe signal. NF54[roGFP2-Orp1]-attB transfectants were significantly oxidized by ML304. CLSM data were composed of 10–20 trophozoites analyzed per experiment for each incubation. Mean values and standard errors of the means (±SEM) are shown for three independent experiments. A one-way ANOVA test with 95% confidence intervals with the Dunnett’s Multiple Comparison Test was applied for statistical analysis of significance (*p < 0.05; **p < 0.01; ***p < 0.001).
