Figure 5 | Scientific Reports

Figure 5

From: Nectin-like molecule-4/cell adhesion molecule 4 inhibits the ligand-induced dimerization of ErbB3 with ErbB2

Figure 5

Interaction of Necl-4 with the domain 3 of ErbB3. (a) FLAG-Necl-4 immunoprecipitation assay. HEK293E cells were co-transfected with various combinations of the indicated plasmids and cultured in suspension. FLAG-Necl-4 was immunoprecipitated using the anti-FLAG mAb, and the samples were subjected to Western blotting using the indicated Abs. Asterisks, full-length or mutants of ErbB3. (b) Reciprocal immunoprecipitation assay. HEK293E cells were co-transfected with various combinations of the indicated plasmids and cultured in suspension. ErbB3-GFP and its mutants were immunoprecipitated using the anti-GFP pAb, and the samples were subjected to Western blotting using the indicated Abs. Arrowheads, full-length or mutants of ErbB3. IB, immunoblotting; IP, immunoprecipitation.

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