Figure 6

TGF-β inhibitors LY2157299 and Decorin could suppress the Laser-induced CNV formation in RPE-choroid-sclera complex of mice. The representative FFA images showed the fluorescence leakage in eye fundus of vehicle (A), TGF-β chemical inhibitor LY2157299 (intraperitoneal injection of 3.0 μg/g BW, B) and TGF-β natural inhibitor Decorin (intravitreal injection of 10 μg in 1 μl PBS, C) treated mice at 2 weeks after Laser photocoagulation (white arrowheads indicated Laser-injured spots). Fluorescein leakage was reduced and no abnormalities were observed in the retinal blood vessels of Laser-injured mice after LY2157299 and Decorin treatments. The isolectin-B4 stained images showed the changes of CNV area in RPE-choroid flat mounts of mice following Laser injury only (D), intraperitoneal injection of PBS (E) and LY2157299 (G), and intravitreal injection of PBS (F) and Decorin (H) at 2 weeks after photocoagulation. Red dotted lines indicate CNV lesions (bar = 100 µm). (I) The areas of CNV lesions were quantified by using digital imaging analysis, and showed that the CNV size was significantly suppressed by both LY2157299 and Decorin treatments when compared with that of controls. *P < 0.05 vs corresponding PBS treatment; n = 5 per group.