Figure 2
From: Interaction of lipopolysaccharides at intermolecular sites of the periplasmic Lpt transport assembly
LptAm is momomeric and forms a complex with LptC. (a) SAXS analysis of LptAm, LptC and LptC:LptAm mixture. P(r) distribution plot is calculated from the X-ray scattering curves of LptAm, LptC and of a LptC:LptAm mixture in a 1:1 ratio. The estimated Rg as well as maximum interatomic distance (Dmax) increase for the LptC-LptA mixture confirming the formation of a complex. (b) SAXS Ab initio envelope determination of LptAm, LptC with their fitted protein structure (see SAXS section in Methods for atomistic models used) inside as ribbons, and LptC-LptAm complex calculated with Damfilt.
