Figure 1 | Scientific Reports

Figure 1

From: The Parkinson’s disease-associated GPR37 receptor interacts with striatal adenosine A2A receptor controlling its cell surface expression and function in vivo

Figure 1

Co-distribution of GPR37 and A2AR in the mouse brain. (A) Cartoon showing the location of the horizontal brain section for the histoblot (left panel). The expected brain regions within the horizontal section (right panel) are indicated: Cb, cerebellum; Hp, hippocampus; Ct, cortex; St, striatum (blue); Call, corpus callosum. (B) Histoblots from horizontal sections (see panel A) of GPR37+/+ and GPR37−/− mouse brain. GPR37 and A2AR were detected using a rabbit anti-GPR37-N antibody (3 μg/ml) and a goat anti-A2AR antibody (3 μg/ml) (see Materials and Methods). (C) The histoblots were scanned and densitometric measurements from six independent experiments were averaged to compare the GPR37 and A2AR protein densities. Since expression was maximal in corpus callosum, normalization was performed assigning it the 100%. Results are expressed as mean ± SEM. Scale bar: 0.4 cm.

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