Figure 1 | Scientific Reports

Figure 1

From: Fast free-of-acrylamide clearing tissue (FACT)—an optimized new protocol for rapid, high-resolution imaging of three-dimensional brain tissue

Figure 1

Pipeline overview of the Fast Free-of-Acrylamide Clearing Tissue (FACT) protocol in comparison with seven other protocols for clearing of mouse brain slices. After perfusion and fixation of brains with paraformaldehyde (PFA) or hydrogel solution, brain slices were allocated into four different clearing solutions with varying sodium dodecyl sulfate (SDS) concentrations and pH. The four subgroups were cleared at two different temperatures.

Back to article page