Figure 5 | Scientific Reports

Figure 5

From: Fast free-of-acrylamide clearing tissue (FACT)—an optimized new protocol for rapid, high-resolution imaging of three-dimensional brain tissue

Figure 5

Three dimensional reconstructions of microglia in 400-µm-thick volumes of cerebral cortex after the different clearing protocols. (A–G) Different colors represent different surface areas of microglia reconstructed by the Imaris Surface algorithm and sorted by Imaris Vantage from white (highest area) to purple (lowest area). The maximum number of visible microglial structures was achieved with the FACT protocol. (H and I) Comparisons of microglial surface area and volume between the various protocols after semiautomatic measurement with the Imaris Surface algorithm. Maximum retention of YFP signal was achieved with the FACT protocol. Scale bars are 100 μm.

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