Figure 7 | Scientific Reports

Figure 7

From: Fast free-of-acrylamide clearing tissue (FACT)—an optimized new protocol for rapid, high-resolution imaging of three-dimensional brain tissue

Figure 7

Three dimensional reconstructions of individual microglia to compare the sensitivity of the FACT protocol with the other protocols. (A–G) Different colors represent different levels of microglial branches reconstructed by the Imaris Filament algorithm and color-sorted by Imaris Vantage from blue (the first level) to white (the last level). Selected microglia from the topmost scanned layers (Z = 0–50 μm) of each XYZ acquisition after the different clearing protocols. (H) Comparison of the proportions of 3D reconstructed microglial filament levels for the seven clearing protocols. FACT and CLARITY - 37 °C protocols resulted in the highest degree of branching. Scale bars are 15 μm.

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