Figure 1

Medial preoptic area (MPOA) subregions identified on Nissl-stained sections and fluorescent images using area markers. (a–c) Schematic drawings showing the MPOA subregions and nuclei of the bed nucleus of the stria terminalis (BNST). The shaded areas in (a–c) were quantitated for gonadal hormone receptor-expressing cells in this study. (d–f) Representative images of Nissl-stained coronal sections along the anterior-posterior axis. (g–i) Representative images of calbindin (green), Neurotensin (magenta) and Penk (light blue). (j–l) Representative images of Vglut2 (green) and oxytocin (magenta). These images are representative of at least six different mice for each image series. Scale bars: 200 μm. 3 v, third ventricle; ac, anterior commissure; ACN, anterior commissural nucleus; ADP, anterodorsal preoptic nucleus; BNSTdm, dorsomedial nucleus of the BNST; BNSTmg, magnocellular nucleus of the BNST; BNSTpr, principal nucleus of the BNST; BNSTv, ventral nucleus of the BNST; cMPOA, central part of the MPOA; dmMPOA, dorsomedial part of the MPOA; MPNc, central part of the MPN; MPNma, anteromedial part of the MPN; MPNmp, posteromedial part of the MPN; MPNp, posterior part of the MPN; MPNvl, ventrolateral part of the MPN; opt, optic tract; PD, posterodorsal preoptic nucleus; SON, supraoptic nucleus; vMPOA, ventral part of the MPOA; vlMPOA, ventrolateral part of the MPOA; VLPO, ventrolateral preoptic nucleus.