Figure 8

Representative fluorescent images of in situ hybridization for neurotransmitters and neuropeptides combined with immunostaining for AR. (a) Gad67. (b) Vglut2. (c) Pdyn. (d) Penk. (e) Cart. (f) Neurotensin. (g) Galanin. (h) Tac1. (i)Tac2. (j) Trh. (k) Orthogonal view of Gad67 and AR positive cells. Each image series is representative of at least three different mice. Arrowheads indicate double positive cells. Scale bars: 50 μm (a–j), 20 μm (k).