Figure 5 | Scientific Reports

Figure 5

From: Expression of IL-33 in ocular surface epithelium induces atopic keratoconjunctivitis with activation of group 2 innate lymphoid cells in mice

Figure 5

Induction of ILC2 in IL33tg mouse cornea. (a) Gating strategy for analysis of FSClowSSClowCD45+Lineage(Lin)−Sca-1+ST2+ ILC2 cells. Note that ILC2 cells are Thy1.2+ and GATA3+. The numbers indicate the percentage of cells in each gate. (b) The proportion of Sca-1+ ST2+ ILC2 from the corneas of 20- to 30-week-old WT and IL33tg mice (Tg) determined by flow cytometry. Values indicate the percentage of ILC2 of Lin− lymphocytes. Data are expressed as means ± SEM (n = 3) *P < 0.0001 (two-tailed t-test). (c,d) The intracellular flow cytometry for IL-5 and IL-13. The cells were gated into CD45+Lin− fraction (c) and CD45+Lin−Sca-1+ST2+ fraction (d). The numbers indicate the percentage of cells in each quadrant. Similar results were obtained in two (a,b) or three (c,d) independent experiments.

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