Figure 6

Induction of basophils in IL33tg mouse cornea. (a) Flow cytometry of cells from the corneas of 20- to 30-week-old WT and IL33tg mice (Tg). Cells were gated on the B220⁻CD3⁻CD4⁻CD8⁻Gr-1⁻NK1.1⁻Siglec-F⁻CD45⁺ fraction. The numbers indicate the proportion of DX5⁺FcεR1⁺ cells. (b) The proportion of DX5⁺FcεR1⁺ determined by flow cytometry. Data are expressed as means ± SEM (n = 3) *P < 0.01 (two-tailed t-test). (c) Immunofluorescence of mMCP-8 in the corneal stroma of WT and IL33tg mice (Tg). The infiltration of mMCP-8⁺ basophils were evident in the corneal stroma of IL33tg mice. Bars, 50 µm. Data are representative of at least six mice. Similar results were obtained in two independent experiments.