Figure 5 | Scientific Reports

Figure 5

From: Overexpression of C-type Natriuretic Peptide in Endothelial Cells Protects against Insulin Resistance and Inflammation during Diet-induced Obesity

Figure 5

Insulin sensitivity in HFD-fed E-CNP Tg mice. (a) Results of glucose tolerance test (GTT), (b) serum insulin levels during GTT, and (c) results of insulin tolerance test (ITT) in HFD-fed mice (age, 20 weeks). (d and e) Activation of Akt phosphorylation in WAT, liver, and muscle after insulin injection (1 U/kg IP, 8 min). (d) Western blot analysis of phosphorylated (Ser473) Akt (pAkt) levels under control (PBS [-]) and insulin-treated ( + ) conditions in WAT, liver, and muscle extracts. (e) Densitometric quantitation of the pAkt:Akt ratio in WAT, liver, and muscle extracts. The gels were run under the same experimental conditions and were cropped to show protein bands corresponding to pAkt, Akt, and tubulin as indicated. n = 12–14 (a and c); n = 7 (b); n = 4 (d and e). *P < 0.05 between Wt and E-CNP mice (a–c) or compared with value for Wt mice without insulin injection (e).

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