Table 1 Summary of dose-response data obtained using TGFβ1 and TGFβ antibody in radioactive and Flamingo assays.

From: Quantitative and organisational changes in mature extracellular matrix revealed through high-content imaging of total protein fluorescently stained in situ

 

TGFβ1

TGFβ antibody

EC50 (ng/mL)

EC50 (nM)

Flamingo

Radioactive

Flamingo

Radioactive

Geometric mean

15.0

3.7

42.6

19.3

Min, max (n)

12.5, 17.7 (4)

2.7, 4.5 (3)

20.8, 64.9 (6)

13.9, 28.0 (4)