Figure 1

Endogenous expression of XPO1 in human ATC samples and cell lines, and effect of silencing of XPO1 in ATC cells. (A) Immunohistochemical analysis for detection of endogenous expression of XPO1 protein in thyroid tumors and benign thyroid tissues. Representative photographs showing nuclear staining (brown color) of XPO1 in different histological subtypes of thyroid carcinoma: papillary, follicular, ATC and benign thyroid (low or no reactivity) (original magnification, ×200; objective ×20). (B) Western blot analysis of ATC cell lines verified that the XPO1 antibody specifically recognizes a band of 123 kDa, corresponding to the size of XPO1 protein. (C) Indirect immunofluorescence assay showing nuclear localization of XPO1 protein in fixed/permeabilized ATC cell lines; DAPI stains nuclei. (D) ATC cells were transduced with either XPO1 shRNA or scrambled shRNA. XPO1 knockdown was confirmed in HTH83 and CAL62 cells by western blotting. GAPDH antibody was used to assure equal loading of lysates. Original and full-length blots are included in the Supplementary Information. XPO1 silencing resulted in decreased cell proliferation of HTH83 and CAL62 as measured using MTT assay. Results represent mean ± SD; n = 4. *P ≤ 0.01; **p ≤ 0.001; ***p ≤ 0.0001(Student’s t-test).