Figure 2

Selinexor significantly suppressed cellular growth and clonogenic ability of human ATC cells in vitro. (A) OGK-M, HTH83, CAL62 and T238 cells were cultured with increasing concentrations of selinexor (0–1,000 nM) for 24 h. Whole-cell lysates were prepared, and XPO1 protein expression was analyzed using western blot analysis. GAPDH was used as an internal control. Cell viability of eight human ATC cell lines. Original and full-length blots are included in the Supplementary Information. (B) Cells were treated with selinexor at indicated concentrations for 72 h, and growth inhibition was measured by MTT assay. Results are expressed as mean value ± SD; n = 4. (C and D) Representative photomicrograph displayed that selinexor treatment decreased clonogenic growth. ATC cells were exposed to indicated concentrations of selinexor for 48 h; cells were washed with drug-free medium and allowed to form colonies for 2 weeks followed by crystal violet staining and quantification. Data represent mean ± SD of four independent experiments done in triplicates. *p ≤ 0.01; **p ≤ 0.001; ***p ≤ 0.0001(Student’s t-test).