Figure 3

Inhibition of XPO1 induced cell cycle arrest and apoptosis of ATC cells in a dose-dependent manner. (A) Silencing of XPO1 in ATC cells resulted in an increased in G1 phase. (B) OGK-M, HTH83, CAL62, and T238 cells were treatment with either different concentrations of selinexor (0–1,000 nM) or diluent control (DMSO), stained with propidium iodide (PI) and analyzed by flow cytometric analysis. Histogram showed the proportion of cells in different phases of cell cycle. Data are presented as the mean of three independent experiments. (C) ATC cells were treated with selinexor at indicated concentrations for 24 h, stained with annexin V-FITC and PI, and subjected to flow cytometric analysis to evaluate the ability of selinexor to induce apoptosis. Histograms represent the percentage of apoptotic cells. Data are presented as mean ± SD of three independent experiments. *p ≤ 0.01; **p ≤ 0.001; ***p ≤ 0.0001for selinexor vs. controls. (D) OGK-M, HTH83, CAL62 and T238 cells were treated with either selinexor (1,000 nM) or DMSO for 24 h. Lysates were analyzed by western blot analysis for the indicated cell cycle and apoptosis proteins (GAPDH, internal loading control). Original and full-length blots are included in the Supplementary Information.