Figure 3 | Scientific Reports

Figure 3

From: BBS4 regulates the expression and secretion of FSTL1, a protein that participates in ciliogenesis and the differentiation of 3T3-L1

Figure 3

FSTL1 accumulates in lysosomes in the absence of BBS4. (A) Control hTERT-RPE1 were co-immunostained for Golgi (top row, Golgin 97 in cyan) or ER (bottom row, Calnexin, cyan) together with FSTL1 (magenta). (B) Analysis of colocalization of FSTL1 with each organelle marker represented as Manders coefficients showing that FSTL1 colocalizes with both organelles. (C) hTERT-RPE1 control and BBS4-KD cells were co-immunostained to study colocalization of FSTL1 (red) and lysosomes (LAMP2 in green). Colocalization is evidenced by colocalized pixels in white. (D) Manders coefficients were determined showing that in BBS4 KD cells, FSTL1 is accumulated in lysosomes. In all cases, scale bars represent 10 µm and ****: P < 0,0001, t-test.

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