Figure 1
From: Studies on the regulatory mechanism of isocitrate dehydrogenase 2 using acetylation mimics
Screening of regulatory lysine in mIDH2 using acetylation mimics. The activity of the KQ mutants compared to that of wild-type mIDH2 is shown. Measuring the activity of each mutant was repeated three times, which served as three technical replicates. The final enzyme concentration was 5 nM, and isocitrate and NADP+ were added at their saturating concentrations of 1 mM. The data represent the means ± SD, and Student’s t-test was used to determine whether differences in values relative to those of wild-type mIDH2 were significant; significance is denoted by an asterisk (p < 0.05).
