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Figure 1

From: Integrative Approach with Electrophysiological and Theoretical Methods Reveals a New Role of S4 Positively Charged Residues in PKD2L1 Channel Voltage-Sensing

Figure 1

Kinetics of voltage-dependent inactivation of human PKD2L1. (a) Inactivation properties of PKD2L1 channels. To determine the voltage dependence of inactivation, currents were evoked by a 40 ms test pulse to +120 mV after 3 s V h displacements (conditioning pulses) from −100 to +140 mV with 20 mV increments. (b) Representative superimposed current traces for steady state inactivation. (c) Steady state inactivation curves. Amplitudes of tail currents are normalised to the maximal peak tail amplitude and plotted against the membrane potential. Points are fitted with a Boltzmann function to yield the voltage for half-maximal inactivation (V 0.5) of 14.7 mV (n = 6). (d) The time course of recovery of peak whole-cell current from inactivation. The pulse protocol was composed of two pulses, one 1 s pre-pulse from −100 to +140 mV, followed by a depolarising pulse to +140 mV with progressively prolonged durations from 0.1 to 20.1 s and then a 40 ms test pulse from −100 mV to +140 mV. The V h was at −100 mV. (e) Representative superimposed current traces for recovery of whole-cell current from inactivation. (f) The normalised value is the result of dividing the test peak currents (I) by the corresponding control peak current (I max). Each symbol represents the mean value of five experiments. Data points are the means ± SEM.

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