Figure 2 | Scientific Reports

Figure 2

From: Integrative Approach with Electrophysiological and Theoretical Methods Reveals a New Role of S4 Positively Charged Residues in PKD2L1 Channel Voltage-Sensing

Figure 2

Voltage dependence of the PKD2L1 channel. (a) Representative whole-cell currents recorded in vector- and PKD2L1-transfected HEK293T cells. The currents elicited by application of step pulses from −100 to +300 mV in 20 mV increments at a holding potential (V h) of −100 mV. The arrowhead indicates the zero current level. (b) Current–voltage relationships (I-V) for instantaneous tail currents at each test pulse recorded from vector- (open circles; n = 7) and PKD2L1-expressing HEK293T cells (filled circles; n = 78). (c) Steady state activation curves. Amplitudes of tail currents normalised to the maximal peak tail amplitude and plotted against the membrane potential. Points are fitted with a Boltzmann function to yield the voltage for half-maximal activation (V 0.5) of +132.1 mV (n = 71).

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