Figure 3
From: Molecular hydrogen increases resilience to stress in mice
Repeated inhalation of hydrogen gas inhibited the chronic stress-induced increase in serum levels of CORT, ACTH, IL-6, and TNF-α. (A) Experimental procedure. After a 5-day adaptation, mice were treated with chronic stress procedure for 28 days. Beginning on day 14, mice were inhaled with mixture gas of H2/O2 [67%/33% (v/v)] or O2/N2 [33%/67% (v/v)] for 3 h daily 0.5 h before stress for 14 days. On day 29, mice were decapitated and serum samples were collected for ELISA analysis. Inhalation of hydrogen gas significantly blocked the increased serum levels of CORT (B), ACTH (C), IL-6 (D), and TNF-α (E) in chronically stressed mice. #P < 0.05 and ##P < 0.01 versus the control group; *P < 0.05 and **P < 0.01 versus the saline-treated CMS group. n = 4–6 per group. CMS, chronic mild stress; CORT, corticosterone; ACTH, adrenocorticotropic hormone; IL-6, interlukin-6; TNF-α, tumour necrosis factor-α.
