Figure 4
From: Molecular hydrogen increases resilience to stress in mice
Repeated inhalation of hydrogen gas in adolescence increased resilience to acute stress in adulthood. (A) Experimental procedure. On the 28th postnatal day (PND), the mice were inhaled mixture gas of H2/O2 [67%/33% (v/v)] or O2/N2 [33%/67% (v/v)] for 1, 3 h daily for 14 days. Behavioural tests were conducted to assess the acute stress-induced depressive- and anxiety-like behaviours. Inhalation of hydrogen gas significantly decreased the floating time in the FST (B) and the immobility time of mice in the TST (C), decreased the latency to feeding (D), but without affecting the total feeding in homecages (E) during the NSF test. *P < 0.05, **P < 0.01 versus the control group. n = 9–10 per group. TST, tail suspension test; FST, forced swimming test. NSF, novelty suppressed feeding test.
