Figure 3
Effects of MLP diet on Ppara gene expression and its target genes in the fasted state Expression differences between control and MLP pups of Ppara and its target genes were tested. (a) Microarray expression data of Ppara and Pparg are shown. Values are 75%-tile normalized (N = 3–4 animals/group). (b) qPCR validation of Ppara and Pparg expression (N = 7 animals/group). Data are shown as mean ± SD. *P < 0.05. (c) PPARα inducible gene sets were prepared based on the PPARα tailored analysis described by Szalowska et al.19. The list of gene components and their biological function are shown in dataset 4. Gene Set Enrichment Analysis20 showing that PPARα-mediated gene upregulation was significantly decreased in MLP pups (normalized enrichment score = 1.43, FDR < 0.022). A heatmap is provided illustrating gene expression levels for each gene in the core enrichment subset (blue, low; red, high). Genes related to lipid metabolism are denoted in blue.
