Figure 2 | Scientific Reports

Figure 2

From: A novel chalcone derivative S17 induces apoptosis through ROS dependent DR5 up-regulation in gastric cancer cells

Figure 2

S17 induced apoptosis in MGC803 cells. (A) S17 induced cell apoptosis of MGC803 cells in a time-dependent manner by Flow Cytometer analysis. (B) MGC803 cells were stained with DAPI solution. Stained nuclei were then observed under a fluorescence microscope using a blue filter (magnification, 400X). The arrow was indicating chromatin condensation. (C) Western blotting assay showed the effects of S17(10 μM) on the expression CF-PARP, CF- caspase3, CF- caspase8 and CF-caspase9. (D) Flow Cytometric analysis demonstrated the effect of the inhibitor of caspases (Z-VAD-fmk, 100 μM) on S17(10 μM)-induced cell apoptosis of MGC803 cells for 48 h.

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