Figure 4
S17 induced cell apoptosis of MGC803 cells involved with extrinsic pathway. Both wild type and DR5−/− MGC803 cells were treated of 10 μM S17 for indicated time. (A) S17 increases DRs protein expression in MGC803 cells at a time-dependent manner. (B) DR5 protein expressions in MGC803 cells were determined by flow cytometer analysis after S17 incubation for 24 hours. (C) Cell viabilities were measured by MTT assay after 30 hours S17 incubation. **p < 0.01 vs. untreated group. (D) The ratios of apoptotic cells were determined by Flow Cytometric analysis after S17 incubation for 48 hours. (E) The expression levels of apoptosis-related proteins were tested by western blotting assay after S17 incubation for 24 hours. (F) Flow Cytometric analysis demonstrates MMP(ΔΨ) decrease after 48 hours treatment of S17.
